Composite

Part:BBa_I716305:Design

Designed by: vincent parker   Group: iGEM07_Berkeley_UC   (2007-07-24)


otsB-RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 831
    Illegal XhoI site found at 840
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 846
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 846
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1927


Design Notes

Digest Bca1144-I716302 (EcoRI, BamHI 2063bp, 816bp. S) Sub into pJD507-pSB1A2C (EcoRI, BglII 2071bp, 9bp. L) Product is I716305


Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References